Infobiochem
Q/A
April 29, 2016
Tryptophan aminotransferase is an enzyme that catalyzes the transamination of L-tryptophan. It requires pyridoxal phosphate as a cofactor and an amino group acceptor alpha-ketoglutarate. Tryptophan aminotransferase catalyzes the first step of indole-3-acetic acid (an auxin) biosynthesis and its product is indole-3-pyruvate. Indole-3-acetic acid is a plant growth …
Infobiochem
Q/A
April 29, 2016
Indole-3-pyruvate decarboxylase is an important enzyme involved in the biosynthesis of indole-3-acetic acid, a plant growth hormone. The indole-3-acetic acid biosynthetic pathway involving indole-3-pyruvate decarboxylase is called an indole-3-pyruvate pathway and indole-3-pyruvate is the key intermediate of this pathway. The first enzyme of this pathway is …
Infobiochem
Q/A
April 29, 2016
Tryptophan decarboxylase is an enzyme that catalyzes the conversion of tryptophan to tryptamine. Tryptamine is a first-reaction intermediate of indole-3-acetic acid (a type of auxin) biosynthesis. Therefore, to study the tryptamine pathway of indole-3-acetic acid biosynthesis, it is necessary to check the activity of the enzymes …
Infobiochem
Q/A
April 28, 2016
The pKa of phosphate buffer can be calculated by using a mathematical expression. Phosphate buffer is an important buffer system used in biochemistry laboratories. Phosphate buffer is essential for experiments involving proteins and enzymes. It maintains the pH of the solution and also prevents a drastic …
Infobiochem
Q/A
April 28, 2016
What is a Salkowski reagent? Salkowski reagent is a formulation that researchers have developed for the determination of the concentration of indole-3-acetic acid. It consists of ferric chloride dissolved in concentrated oxidizing acid. It was originally developed by Salkowski in 1885 for the colorimetric determination of …
Infobiochem
Q/A
April 28, 2016
Ehmann’s reagent is a mixture of Ehrlich reagent and Salkowski reagent in a ratio of 1:3 (v/v). It is used to detect indole intermediates during thin-layer chromatography. This reagent is greatly helpful to detect the presence of different types of indole intermediate. However, standard indole compounds …
Infobiochem
Q/A
April 28, 2016
Bradford assay is a protein quantification protocol developed by Marion Mckinley Bradford in 1976. It gives an easy way to estimate the protein concentration of a solution using a standard curve generated by the use of known concentrations of a protein. First, you need to prepare …
Urea and formamide are used as denaturants in Denaturing Gradient Gel Electrophoresis (DGGE). In DGGE, urea, and formamide denature the DNA fragments by disrupting the Hydrogen bonding between the nitrogenous base pairs. As shown in the figure above, urea and formamide form a Hydrogen bond with nitrogenous bases …
Infobiochem
Q/A
April 9, 2016
Ribosomes, protein synthesizing apparatus of the cell. Before answering where does a peptide bond form, I’m sure you know where does a protein synthesized inside a cell. Proteins are synthesized in the ribosomes located in the cytosol of the cell. A ribosome is a protein (practically …
