This is the best phosphate buffer calculator available elsewhere online. I have developed it just for you so that you can calculate the required amount of individual components. Actually, you can manually calculate the amounts of individual components of a phosphate buffer but that will take a lot of time.
Therefore, to save you time in such a lengthy calculation, I have developed this phosphate buffer calculator. You just need to enter the necessary information in the form and you will automatically get the amounts of individual components that you need. To know more about the manual calculation of phosphate buffer components, visit this; How to prepare 100 ml of 50 mM phosphate buffer of pH 6.8?
Phosphate buffer is an important buffer that we use in biochemistry laboratories. It is often used to extract and isolate proteins and enzymes that have optimal pH of around 7. That is because the buffering capacity of a phosphate buffer is pH 7.21 ± 1 within which most of the proteins and enzymes are more stable.
For a phosphate buffer, you will need two components; dihydrogen phosphate ion and hydrogen phosphate ion. Actually, you can use either sodium phosphate or potassium phosphate buffer for the preparation of a phosphate buffer.
Sometimes, researchers also use a combination of sodium and potassium phosphate for the preparation of phosphate buffer to balance the sodium and potassium ion requirements. Anyway, both work well as long as you have prepared the buffer in the right way.
Phosphate buffer calculator available elsewhere
Though there are many other online phosphate buffer calculators such as the phosphate buffer calculator provided by the Centre for Proteome Research, Liverpool, Buffers in Molecular Biology by Tripod.com buffer calculator by Biomol.net, Phosphate buffer calculator provided by Altervista.org and work well with various conditions. However, they take some time to give you the results. You click on the calculate button and wait for a while until you see the results.
Considering the facts that I had observed on these other calculators, I have developed this online phosphate buffer calculator. I bet, you will like it more than any other calculator that you have seen elsewhere.
It is so simple in the sense that, as soon as you finish entering the data, you will get your result. You don’t need to click on the calculate button or show the result button or anything else. It’s real-time and faster than other calculates.
All you need is the amounts of individual components of the phosphate buffer. As soon as you get these data here (expressed in grams), you can proceed with preparation. Weigh the required amount of each component and put it in a volumetric flask. Add some distilled water and dissolve all the solutes. Once the solution is clear, add more distilled water to make the desired volume and mix it again. Now, you are done making a phosphate buffer of a specific concentration and pH. Thank you for using our phosphate buffer calculator.
I tried your buffer calculator and got a completely different answer from the other (http://serge.engi.tripod.com/MolBio/Buffer_cal.html). That makes no sense to me.
Na2HPO4 142 mw
K2HPO4 136 mw
5mM pH 6.5
Yours: 0.116/0.569 g
Theirs: 0.214/0.475 g
Let me first tell you that you are confused with these two different calculators. One (available on this website) gives you required amount of the components in gm to be used to make the buffer of desired concentration and pH and the other you compared with, gives you the concentration of the individual components of a buffer of desired pH and concentration.
From that value of concentration, you will still need to calculate the amount in gram to be used to prepare the buffer of desired pH and desired Concentration. And at last, to make a phosphate buffer you need acid-base components that can be of any of these four types; NaH2PO4-Na2HPO4/KH2PO4-K2HPO4/NaH2PO4-K2HPO4/KH2PO4-Na2HPO4.
Lets assume you used NaH2PO4 (acid component with m.wt:119.98) and Na2HPO4 (base component with m.wt.141.96) and you want to prepare 100ml of 5mM phosphate buffer of pH6.5 the calculation using our calculator would give you this; NaH2PO4: 0.05gm and Na2HPO4: 0.012gm. These are the amounts that you will need to mix to make 100 ml of Phosphate buffer of pH 6.5 and strength 5mM. Supposed you used another website as you said you will endup with these two values NaH2PO4: 0.41907 g/L and Na2HPO4: 0.21396 g/L. These are the concentrations of individual components not the amounts in gram to be used to prepare the designated buffer.
From these last two values you will still have to calculate the weiht in gm to be used to prepare the buffer using molarity-molecular weight-weight formula. Hope you understood it now.