Classification of the lipids Lipids are classified into two classes; saponifiable lipids and non-saponifiable lipids. In a saponifiable lipid family, there are two types of lipids Glycerol Esters and Non-glycerol Esters. Glycerol esters include triacylglycerols and phosphoglycerols while non-glycerol esters include two classes of lipids; waxes …
Proteins associated with or embedded in a biological cell membrane are called membrane proteins. Membrane proteins are of two types. Integral Proteins Proteins that are embedded deeply in the plasma membrane are integral proteins. They are held inside the membrane by hydrophobic interaction. Integral proteins may …
An introduction to the glycoproteins The glycoprotein is a type of glycoconjugate. It is a biological molecule that contains proteins as well as carbohydrates (especially oligosaccharides) linked to each other through the covalent linkage between the sugar unit of oligosaccharide and a side chain of certain types …
What is SDS PAGE? SDS PAGE or Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis is an analytical tool widely used to analyze protein mixtures qualitatively as well as quantitatively. However, we can also use it to determine the molecular weight of an unknown protein using the SDS …
What are APS and TEMED? Before jumping into the uses of the APS and TEMED in SDS PAGE, let’s know more about the individual components. APS is the ammonium persulfate while TEMED is the N, N, N’, N’-tetramethyl ethylene diamine. In the same manner, SDS is …