Acetate buffer calculator This is the best acetate buffer calculator available online. I’m sure you will find it very much useful. An acetate buffer has many uses, for example, it is can be used in nucleic acid precipitation and protein crystallization. Furthermore, it can also be …
Infobiochem
Q/A
January 28, 2018
To know how to prepare 50mM Phosphate buffer of pH 6.8, first, you need to know three factors that are needed to prepare a phosphate buffer (in fact any buffer system). These are the concentration of the buffer, pH value, and final volume of the buffer …
This is an online molarity calculator that I have developed to calculate molarity, mass, and volume. The formula used in this online molarity calculator uses simple molarity formula but with little modifications in unit conversion. Molarity is a way of expressing the concentration of a …
This is the best phosphate buffer calculator available elsewhere online. I have developed it just for you so that you can calculate the required amount of individual components. Actually, you can manually calculate the amounts of individual components of a phosphate buffer but that will take …
Infobiochem
Q/A
June 19, 2017
Biochemistry students often ask themselves one of the common questions; how to memorize structures of all 20 amino acids. However, using some tips, it’s possible to memorize all of the 20 standard amino acids. It’s not that hard for students to do that. You just need …
The extraction of DNA from agarose gel has many downstream applications in molecular biology research work and biomedical research. For instance, we often need to sequence or clone the DNA fragments obtained either by PCR or restriction digestion. Therefore, it is necessary to have a pure …
Electroelution is one of the numerous methods available for DNA extraction from agarose gel. Here, I have described some of the specialized electroelution apparatus. These can easily be used to isolate DNA from the agarose gel. Though these apparatuses may be expensive to buy they offer …
Agarose gel extraction can be made easy by using commercially available gel extraction kits or advanced electrophoretic apparatuses that allow the visualization of DNA bands during the electrophoresis. Conventional methods include dialysis of the sliced gel containing DNA by applying an electric current while some other methods include spin columns and Sephadex …
Here, I have described different types of commercially available gel extraction kits for gel excision. These are listed below in a table summarizing the most important comparative information about different kits. These commercially available gel extraction kits can easily be utilized for DNA gel excision. The …
