The extraction of DNA from agarose gel has many downstream applications in molecular biology research work and biomedical research. For instance, we often need to sequence or clone the DNA fragments obtained either by PCR or restriction digestion. Therefore, it is necessary to have a pure …
Electroelution is one of the numerous methods available for DNA extraction from agarose gel. Here, I have described some of the specialized electroelution apparatus. These can easily be used to isolate DNA from the agarose gel. Though these apparatuses may be expensive to buy they offer …
Agarose gel extraction can be made easy by using commercially available gel extraction kits or advanced electrophoretic apparatuses that allow the visualization of DNA bands during the electrophoresis. Conventional methods include dialysis of the sliced gel containing DNA by applying an electric current while some other methods include spin columns and Sephadex …
Here, I have described different types of commercially available gel extraction kits for gel excision. These are listed below in a table summarizing the most important comparative information about different kits. These commercially available gel extraction kits can easily be utilized for DNA gel excision. The …
Here, I have described some of the recently developed portable agarose gel electrophoresis systems with the built-in safe imager. The built-in imager or illuminator helps us visualize the DNA migration in real time while doing electrophoresis. These setups have been designed in such a way that …
