Infobiochem
Q/A
April 28, 2016
Bradford assay is a protein quantification protocol developed by Marion Mckinley Bradford in 1976. It gives an easy way to estimate the protein concentration of a solution using a standard curve generated by the use of known concentrations of a protein. First, you need to prepare …
Urea and formamide are used as denaturants in Denaturing Gradient Gel Electrophoresis (DGGE). In DGGE, urea, and formamide denature the DNA fragments by disrupting the Hydrogen bonding between the nitrogenous base pairs. As shown in the figure above, urea and formamide form a Hydrogen bond with nitrogenous bases …
Infobiochem
Q/A
April 9, 2016
Ribosomes, protein synthesizing apparatus of the cell. Before answering where does a peptide bond form, I’m sure you know where does a protein synthesized inside a cell. Proteins are synthesized in the ribosomes located in the cytosol of the cell. A ribosome is a protein (practically …
Everybody knows HDL cholesterol or High-Density Lipoprotein is good for health while LDL or Low-Density Lipoprotein is bad. However, HDL is not always good for health. One recent study shows HDL can be bad for heart health. Before getting into the topic, I would like to …
