Infobiochem
Q/A
June 19, 2017
Biochemistry students often ask themselves one of the common questions: how to memorize the structures of all 20 amino acids. However, using some tips, it’s possible to memorize all of the 20 standard amino acids. It’s not that hard for students to do that. You just …
The extraction of DNA from agarose gel has many downstream applications in molecular biology research work and biomedical research. For instance, we often need to sequence or clone the DNA fragments obtained either by PCR or restriction digestion. Therefore, it is necessary to have a pure …
Electroelution is one of the numerous methods available for DNA extraction from agarose gel. Here, I have described some of the specialized electroelution apparatus. These can easily be used to isolate DNA from the agarose gel. Though these apparatuses may be expensive to buy they offer …
Agarose gel extraction can be made easy by using commercially available gel extraction kits or advanced electrophoretic apparatuses that allow the visualization of DNA bands during the electrophoresis. Conventional methods include dialysis of the sliced gel containing DNA by applying an electric current while some other methods include spin columns and Sephadex …
Here, I have described different types of commercially available gel extraction kits for gel excision. These are listed below in a table summarizing the most important comparative information about different kits. These commercially available gel extraction kits can easily be utilized for DNA gel excision. The …
Here, I have described some of the recently developed portable agarose gel electrophoresis systems with the built-in safe imager. The built-in imager or illuminator helps us visualize the DNA migration in real time while doing electrophoresis. These setups have been designed in such a way that …
Insulin receptor (IR) is a member of receptor tyrosine kinases having a critical role in the insulin-mediated regulation of central metabolism, growth, and development. It is a tetramer of (αβ)2 type tyrosine kinase. Each αβ heterodimer is composed of an extracellular ligand-binding α subunit and transmembrane-spanning …
Though proteins can be stored with cosolvents such as salts and polyols to enhance their stability and activity for biological and biotechnological applications, however, the mechanisms for protein stability remain elusive. Generally, these cosolvents promote the shifting of protein conformations towards a more compact form and …
Biogenic amines are the amines found in the biological system with many vital functions and roles. First, let me describe what is an amine. Amines are organic compounds containing an amino group as a functional group. They are basically considered as a derivative of ammonia in …
Infobiochem
Q/A
October 17, 2016
Before telling you what are the main functions of polysaccharides in plants, I would like to mention polysaccharides first. Polysaccharides are carbohydrates that mean hydrates of carbon. The simplest formula for carbohydrates is Cn(H2O)n, where n can be 3 or more than 3. The simplest carbohydrates are …
Hormones are the first messengers that trigger the various biological and physiological processes inside the body. Among the various types of hormones, steroid hormones are important because of their hydrophobic nature. The main precursor of the steroid hormones is cholesterol which is why they are hydrophobic …
